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目的观察日本血吸虫(Schistosoma japonicum,Sj)童虫细胞型免疫原和童虫细胞碎片免疫原免疫小鼠后在免疫部位滞留的动态变化。方法昆明鼠实验组A和B经大腿肌肉分别注射107日本血吸虫原代童虫细胞(pJCs)和相当剂量的童虫细胞碎片(JCFs),对照组C注射PBS。分别在注射后1、3、6、9和12 d,各组随机处理4或2只小鼠,从注射部位定量获取肌肉组织,用Western blot法和免疫组化法观察、比较两种类型抗原滞留的动态变化;用组织病理学方法观察发生的炎症反应。结果Western blot显示,A组在注射后各时间点均检测到分子质量单位为75 ku的特异性抗原条带,B组在注射后第6 d该条带即消失,C组未检出该条带。免疫组化检测结果与Western blot相符。组织病理学检查结果显示,A组在免疫后第12 d仍可见肌细胞间隙内大量炎性细胞浸润,B组在免疫后第12 d炎性细胞基本消失,C组未见炎性反应。结论血吸虫童虫细胞型免疫原较童虫细胞碎片免疫原在免疫部位可滞留更长时间,引起更持久的炎性反应,这可能是其诱生高保护性抗血吸虫病免疫的重要机制之一。
OBJECTIVE: To observe the dynamic changes in the immune sites of mice immunized with Schistosoma japonicum (Sj) Schistosoma japonicum (Sj) cell-type immunogen and Schistosoma fragilis immunogen. Methods Kunming mice A and B were injected with 107 primary Schistosoma japonicum (pJCs) and considerable amounts of Schistosoma japonicum (JCFs) through the thigh muscles, respectively. The control group C was injected with PBS. At 1, 3, 6, 9 and 12 days after injection, 4 or 2 mice were randomly treated in each group. Muscle tissues were obtained quantitatively from the injection site. Western blot and immunohistochemistry were used to observe the effects of two types of antigen Dynamic changes of retention; observed by histopathological inflammatory response occurred. Results Western blot showed that the specific antigen band of 75 ku in molecular mass units was detected in group A at each time point after injection. In group B, the band disappeared on the 6th day after injection and was not detected in group C band. The results of immunohistochemistry were consistent with Western blot. Histopathological examination showed that a large number of inflammatory cell infiltration was found in muscle interstitium of group A on day 12 after immunization. Inflammatory cells disappeared in group B on the 12th day after immunization, and no inflammatory reaction was observed in group C at 12 d. Conclusion Schistosoma schistosoma cell-type immunogen may stay in the immune site for a longer time than the Schistosoma fragilis immunogen, causing a more permanent inflammatory reaction, which may be one of the important mechanisms of immunization against high protective anti-schistosomiasis .