论文部分内容阅读
目的 了解山东地区输血后丙型肝炎 (Post transfusedhepatitisC ,PTH C)患者庚肝病毒 (hepatitisGvirus,HGV)感染的状况及HGV部分基因核苷酸序列。方法 应用RT PCR法检测HGVRNA ,并对阳性扩增的产物采用PCR技术片段直接克隆法测定。结果 从 86例PTH C患者血清中检出HGVRNA阳性者 31例 (36 % ) ,其中一例患者HGVNS5区部分核苷酸序列与美国原始株 (u44 40 2 )和另一例日本株 (d872 5 5 )核苷酸同源性比较分别为 86 %和 84%。结论证实山东地区PTH C患者存在着HGV感染和HGV HCV混合感染 ,是否该地区存在着不同亚型或HGVRNA阳性携带者 ,有待进一步证实。
Objective To investigate the status of hepatitis G virus (HGV) infection and the nucleotide sequence of some HGV genes in posttransfused hepatitis C (PTH C) patients after transfusion in Shandong province. Methods The HGV RNA was detected by RT-PCR and the product of positive amplification was determined by PCR cloning method. Results Totally 31 cases (36%) of HGV RNA positive were detected in the sera of 86 cases of PTH C patients. One of them had partial nucleotide sequence of HGVNS5 region compared with that of u44 40 2 and d872 5 5, Nucleotide homology was 86% and 84% respectively. Conclusion It is confirmed that HGV and HGV HCV co-infection exist in patients with PTH C in Shandong Province. Whether there are different subtypes or HGVRNA positive carriers in this area remains to be confirmed.