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目前尚无有效可靠的抗应激药物.传统的补益中药六味地黄汤(LW)对多种类型应激所致内分泌和免疫平衡失调具有明显调节作用,但目前已上市的LW成药缺乏可靠的质量控制方法,其临床疗效的可靠程度难以估测.本课题组在前期研究中以免疫和内分泌活性评价为导向,从LW中追踪分离并组成了质量可控且安全性好的六味地黄苷糖(LW-AFC).本文观察了悬吊应激雌性小鼠生殖内分泌及免疫系统的变化及LW-AFC的影响.结果表明,悬吊应激小鼠皮质酮升高,动情期缩短、动情间期延长,垂体LH下降,动情间期血清E2升高;脾细胞培养上清IgG含量明显降低;口服LW-AFC能显著降低血清皮质酮,升高垂体LH,且LW-AFC能明显降低动情间期血清E2,并能明显增强脾细胞IgG分泌能力.这提示,悬吊应激可导致雌性小鼠生殖内分泌功能紊乱以及免疫功能下降,口服LW-AFC具有明显调节作用,对应激所致生殖内分泌功能紊乱以及免疫功能下降具有潜在防治作用.
There is no effective and reliable anti-stress drugs.Traditional Chinese herbal medicine Liuweidihuang Decoction (LW) has a significant regulatory effect on various types of stress-induced endocrine and immune balance disorders, but LW medicines currently on the market are lack of reliable quality Control method, the clinical efficacy of the degree of reliability is difficult to estimate.This group in the previous study to evaluate the immune and endocrine activity-oriented, from the LW trace separation and composition of the quality controllable and safe Liuweidu sugar glycosides ( LW-AFC) .The changes of reproductive, endocrine and immune system and the effect of LW-AFC in female mice with suspension stress were observed.The results showed that the corticosterone in suspension stress mice was increased, the estrus period was shortened, Prolongation, decrease of pituitary LH and increase of serum E2 during estrus; IgG content of spleen cell culture supernatant decreased obviously; Oral administration of LW-AFC could significantly decrease serum corticosterone and elevate pituitary LH, and LW-AFC could significantly reduce the duration of estrus Serum E2, and can significantly enhance the ability of spleen cells IgG secretion.This suggests that the suspension stress can cause reproductive and endocrine dysfunction in female mice as well as decreased immune function, oral LW-AFC has a significant regulatory effect on the stress-induced reproduction Secretory dysfunction and decreased immune function a potential preventive effect.