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目的:探讨乙肝病毒外膜大蛋白与HBV-DNA、HBeAg之间的关系及在判断病毒复制中的临床应用价值。方法:分别采用ELISA法检测HBV-LP、HBeAg,实时荧光定量PCR法检测HBV-DNA,并且分析比较三者之间的阳性率及相关性。结果:在222例慢性乙肝患者中,HBV-DNA的拷贝数与HBV-LP的吸光度值具有良好的正相关性(r=0.640,P<0.05);HBV-DNA的阳性率为74.8%,HBV-LP的阳性率为86.0%,HBeAg的阳性率为41.4%;三者之间的阳性率比较有显著性差异(P<0.05),阳性率HBV-LP>HBV-DNA>HBeAg;在166例HBV-DNA阳性的慢性乙肝患者中,HBV-LP的阳性率为91.6%,HBeAg的阳性率为54.2%,两者比较有显著性差异(P<0.05);在92例HBeAg阳性的慢性乙肝患者中,HBV-LP的阳性率与HBV-DNA比较无显著性差异(P>0.05),但在130例HBeAg阴性的慢性乙肝患者中,HBV-LP的阳性率与HBV-DNA比较有显著性差异(P<0.05),前者阳性率高于后者。结论:作为乙肝病毒在体内的复制指标,HBV-LP在血清学水平能更准确可靠的反映其在体内的复制情况,特别是在HBeAg阴性的慢性乙肝患者中能更准确敏感的反映体内病毒复制情况,值得推广使用。
Objective: To investigate the relationship between hepatitis B virus outer membrane protein and HBV-DNA, HBeAg and its clinical value in judging virus replication. Methods: HBV-LP and HBeAg were detected by ELISA and HBV-DNA by real-time fluorescence quantitative PCR respectively. The positive rate and correlation between them were analyzed. RESULTS: In 222 patients with chronic hepatitis B, the copy number of HBV-DNA had a positive correlation with the absorbance of HBV-LP (r = 0.640, P <0.05). The positive rate of HBV-DNA was 74.8% LP positive rate was 86.0%, HBeAg positive rate was 41.4%; the positive rate of the three was significantly different (P <0.05), the positive rate of HBV-LP> HBV-DNA> HBeAg; in 166 cases The positive rate of HBV-LP was 91.6% and the positive rate of HBeAg was 54.2% in patients with HBV-DNA positive chronic hepatitis B (P <0.05). In 92 patients with HBeAg-positive chronic hepatitis B , The positive rate of HBV-LP was not significantly different from that of HBV-DNA (P> 0.05). However, the positive rate of HBV-LP in 130 HBeAg-negative chronic hepatitis B patients was significantly different from that of HBV-DNA (P <0.05), the former positive rate higher than the latter. Conclusion: As an in vivo replication marker of hepatitis B virus, HBV-LP can more accurately and reliably reflect the in vivo replication of HBV-LP, especially in patients with HBeAg-negative chronic hepatitis B, which can reflect more accurately and in vivo virus replication Situation, it is worth promoting the use.