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背景与目的:选用二甲基苯并蒽(7,12-dimethylbenz(a)anthracene,DMBA)诱发的金黄色地鼠口腔癌模型,进行了姜黄素对口腔癌的预防作用研究,并探讨其防癌机制。材料与方法:试验设阳性对照组(局部涂0.5%DMBA,每周3次,共14周)、二个姜黄素组(在涂DMBA2周前开始分别涂5μmol/L和10μmol/L姜黄素至14周实验结束)和阴性对照组(仅涂石蜡油)。结果:10μmol/L姜黄素处理显著降低了口腔肿瘤发病率和癌发病率,5μmol/L对肉眼肿瘤数目和体积、异常增生及癌数目的抑制分别降低了33.8%、36.3%、37.6%和29.0%。10μmol/L分别降低了46.0%、63.7%、44.7%和37.0%。此外,姜黄素处理均抑制了单纯增生和异常增生组织的微核形成和单纯增生、异常增生和癌组织的Brdu增殖指数。结论:姜黄素对DMBA诱发的地鼠口腔癌有预防作用,其机制与保护DNA损伤、抑制细胞增殖有关。
BACKGROUND & AIM: To study the preventive effect of curcumin on oral cancer in golden hamster oral cancer model induced by 7,12-dimethylbenz (a) anthracene (DMBA) Cancer mechanism. MATERIALS AND METHODS: A positive control group (topical 0.5% DMBA, 3 times a week for 14 weeks) and two curcumin groups (5 micromol / L and 10 micromol / L curcumin 14 weeks end of experiment) and negative control group (only paraffin oil). Results: The treatment with 10μmol / L curcumin significantly reduced the incidence of oral cancer and the incidence of cancer. The inhibitory effect of 5μmol / L on the number and volume of gross tumors, abnormal proliferation and the number of cancers decreased by 33.8%, 36.3%, 37.6% and 29.0%, respectively %. 10μmol / L decreased by 46.0%, 63.7%, 44.7% and 37.0% respectively. In addition, curcumin treatment inhibited micronuclei formation and proliferation, hyperplasia and abnormal proliferation of Brdu in proliferating and hyperplasia tissues. CONCLUSION: Curcumin can prevent DMBA-induced hamster oral cancer and its mechanism is related to the protection of DNA damage and inhibition of cell proliferation.