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由于许多蠕虫具有多种抗原,以致长期以来都认为非特异性是寄生虫病血清学诊断中的一个问题。猪带绦虫囊虫病患者血清在用包虫抗原作血清学试验时,常产生高滴度的结果。不过,用定性的标准能大大提高特异性〔如用免疫电泳试验鉴定包虫特异的弧5(arc5)〕;经过对500多例患者作临床观察,已确认根据弧5的存在可以确诊细粒棘球绦虫、多房棘球绦虫和E.Vogeli的感染。但是,最近又发现,囊虫病患者血清也出现弧5,这样就对弧5确诊包虫病的概念提出了怀疑。本文试图用包虫抗原与囊虫抗原来检测两种患者血清的间接血凝试验(IHAT)的滴度,并用包虫抗原作免疫电泳试验(IEPT)和双向扩散试验,观察出现沉淀带的数目和类型(包括弧5),以鉴别包虫病和囊虫病。取病理学确诊的包虫病和囊虫病患者血清,并记录病史和临床表现。在IHAT中,血清作2倍稀释,自1∶32～1∶4096,用单体的包虫囊液为抗原作
Since many worms have multiple antigens, the nonspecificity has long been recognized as a problem in the serological diagnosis of parasitic diseases. Serum from patients with Taenia solium have often produced high titers when used as a serological test for hydatid disease. However, using qualitative criteria can greatly improve the specificity (such as the use of immunoelectrophoresis test identified hydatidarum-specific arc 5 (arc5)]; after more than 500 patients for clinical observation, has confirmed the presence of arc 5 can be confirmed by the presence of fine particles Echinococcus, Echinococcus multilocularis and E.Vogeli infection. However, it has also recently been found that the serum of patients with cysticercosis also appears arc 5, thus raising doubts about the concept of arc 5 in the diagnosis of hydatid disease. This article attempts to detect the titer of indirect hemagglutination test (IHAT) of the two patients’ sera using hydatid antigen and cysticerminal antigen. The hydatid cyst antigen was used for immunoelectrophoresis test (IEPT) and two-way diffusion test to observe the number of precipitated bands And type (including arc 5) to identify echinococcosis and cysticercosis. Pathological diagnosis of echinococcosis and cysticercosis patients sera, and record the history and clinical manifestations. In IHAT, the serum was diluted 2-fold, ranging from 1:32 to 1:4096 with the hydatid fluid of the monomer as antigen