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大鼠经每日腹腔内注射50mg/kg的丙烯酰胺共10次后,出现双后肢瘫痪等亚急性中毒症状。于自染毒起第5、12、21、36和50天分别对大鼠坐骨神经、小脑和血清中β-葡萄糖醛酸酶(β-G)活性进行测定,发现在第36天(停止染毒后24天)中毒症状明显好转时,坐骨神经中β-G活性显著升高,为对照组的167.15％(P<0.01),第50天时仍持续在高水平,为对照组164％(P<0.01)。小脑中β-G活性仅轻度升高,与对照组比较并无显著性差异。血清中β-G活性未见明显改变。β-G活性测定方法简易、稳定、是观察受损神经修复的灵敏生化指标。
Rats were injected intraperitoneally with 50mg / kg of acrylamide 10 times a day, with paralysis of the hind limbs and other subacute poisoning symptoms. The activities of β-glucuronidase (β-G) in rat sciatic nerve, cerebellum and serum were measured at the 5th, 12th, 21st, 36th and 50th day after exposure respectively and found that on the 36th day (P <0.01). The β-G activity in the sciatic nerve was significantly increased at the end of the first 24 days (P <0.01), and remained high at the 50th day ). Β-G activity in cerebellum increased only slightly, compared with the control group no significant difference. Serum β-G activity no significant change. β-G activity determination method is simple and stable, is to observe the sensitivity of damaged nerve biochemical indicators.