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为阐明小菜蛾Plutella xylostella不同地理种群的遗传多样性,应用ISSR技术对我国小菜蛾8个地理种群的遗传多样性进行了研究分析。15条引物扩增出395条ISSR条带,其中多态性条带占89.11%,全部个体显示了各自独特的ISSR图谱。ISSR标记的遗传多样性分析结果表明:小菜蛾无论在物种水平上(P=89.11%,H=0.2706,I=0.4286),还是在种群水平上(P=88.80%,H=0.2759,I=0.4349)都表现出较高的遗传多样性。其中,北京南口种群内遗传变异最大,海南海口和甘肃兰州种群内遗传变异最小,南方地区(云南、湖北)小菜蛾种群遗传多样性明显高于北方地区(北京、天津、山东、黑龙江、甘肃)种群。据种群变异来源分析,有5.66%的遗传变异来自种群间,94.34%的变异来源于种群内(N_m=8.3399),不同地理种群间没有明显的遗传分化。本文有关小菜蛾不同地理种群基因流动和遗传变异的研究为小菜蛾抗药性的控制及田间种群的综合防治提供了有价值的分子生物学依据。
In order to elucidate the genetic diversity of Plutella xylostella population in different geographic populations, genetic diversity of eight diamondback moth Plutella xylostella populations in China was studied using ISSR technique. 15 primers amplified 395 ISSR bands, of which 89.11% of the polymorphic bands, all individuals showed their own unique ISSR patterns. The genetic diversity of ISSR markers indicated that the diamondback moths were resistant to the disease at the species level (P = 89.11%, H = 0.2706, I = 0.4286) or at the population level (P = 88.80%, H = 0.2759, I = 0.4349 ) Showed higher genetic diversity. Among them, the genetic variation within the populations of the south of Beijing was the largest, while that of the populations of Hainan and Gansu was the lowest. The population genetic diversity of the diamondback moth, Plutella xylostella, in the southern areas (Yunnan and Hubei) was significantly higher than that in the northern areas (Beijing, Tianjin, Shandong, Heilongjiang and Gansu) Population. According to the analysis of source of population variation, 5.66% of the genetic variation came from the population, 94.34% of the variation came from within the population (N_m = 8.3399), and there was no obvious genetic differentiation among different geographic populations. In this paper, the studies on gene flow and genetic variation among different geographical populations of Plutella xylostella provide a valuable molecular basis for controlling the resistance of Plutella xylostella and controlling the population in the field.