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目的探讨建立改良的盲肠结扎穿刺法制备大鼠脓毒症模型。方法将20只雄性SD大鼠随机分为假手术组与脓毒症模型组,每组10只,分别进行假手术及盲肠结扎穿刺手术。各组大鼠分别于造模前、后不同时间点股静脉取血1ml,进行血常规和肌酐、尿素氮、谷丙转氨酶、谷草转氨酶检测,同时观察大鼠术后24h存活率。结果 24h生存率:假手术组大鼠为100%,模型组大鼠为20%;与假手术组相比,模型组大鼠白细胞计数术后2h显著性降低(P<0.05);肌酐、尿素氮水平6h显著升高(P<0.05);谷丙转氨酶与谷草转氨酶8h均显著升高(P<0.05,P<0.01)。结论改良的盲肠结扎穿刺法能建立稳定的大鼠脓毒症模型。
Objective To investigate the establishment of a modified cecal ligation and puncture method for the preparation of rat sepsis model. Methods Twenty male Sprague-Dawley rats were randomly divided into sham operation group and sepsis model group, with 10 rats in each group. Sham operation and cecal ligation were performed respectively. Rats in each group were given 1 ml blood before and after modeling, respectively, for blood routine examination, creatinine, urea nitrogen, alanine aminotransferase, and aspartate aminotransferase. The survival rate of rats after 24 hours of operation was also observed. Results 24h survival rate was 100% in sham operation group and 20% in model group. Compared with sham operation group, white blood cell count of model group decreased significantly at 2 hours after operation (P <0.05) Nitrogen levels increased significantly at 6h (P <0.05), while alanine aminotransferase and aspartate aminotransferase increased significantly at 8h (P <0.05, P <0.01). Conclusion Improved cecal ligation and puncture can establish a stable rat sepsis model.