论文部分内容阅读
建立了微乳液毛细管电动色谱快速测定解热镇痛药中非那西丁、氨基比林和咖啡因的新方法。采用由乙酸乙酯-十二烷基硫酸钠(SDS)-正丁醇-硼砂缓冲液组成的微乳液体系,以氯霉素为内标,3种有效成分在2.5 min内完成分离,峰面积相对标准偏差(RSD)在1.2%～1.6%之间,回收率在95.6%～104.0%之间。实验考察了缓冲溶液的浓度、pH值、SDS浓度以及助表面活性剂的种类、含量对分离测定的影响。该法可用于实际样品分析。
A new method for the rapid determination of phenacetin, aminopyrine and caffeine in antipyretic and analgesic drugs by microemulsion capillary electrokinetic chromatography was established. The microemulsion system consisted of ethyl acetate-sodium dodecyl sulfate (SDS) -butanol-borax buffer was used. Chloramphenicol was used as the internal standard and the three active components were separated within 2.5 minutes. The peak area The relative standard deviations (RSDs) ranged from 1.2% to 1.6%, and the recoveries ranged from 95.6% to 104.0%. The effects of buffer concentration, pH value, SDS concentration and the kind and content of cosurfactant on the separation were investigated experimentally. This method can be used for actual sample analysis.