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目的探讨消黄方对进展期二甲基亚硝胺(DMN)诱导大鼠肝硬化的干预作用。方法 Wistar雄性大鼠34只,随机分为正常组(10只)和模型组(24只)。腹腔注射DMN4周制备肝硬化模型。造模2周末取正常和模型大鼠各3只做药前观察,其余模型大鼠随机分为消黄方组、三氯化钆组和模型对照组(各7只);消黄方组于继续造模同时每天给予消黄方煎出液灌胃2周;三氯化钆组予三氯化钆每周2次(7mg/kg)尾静脉注射。4周末处死全部大鼠。观察大鼠的死亡情况,体重、肝脏大体形态、肝功能、肝脏及脾脏重量、肝脏组织学变化等。结果 DMN造模2周,大鼠肝脏呈现明显的纤维化,造模4周大鼠肝硬化形成。与正常组相比,血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)活性及总胆红素(TBiL)含量随造模时间呈梯形增加;而血清总蛋白(TP)和白蛋白(Alb)含量随模型进展逐渐降低;4周时与模型对照组相比,消黄方组显著降低血清ALT、AST、ALP活性及TBiL含量(P<0.05或P<0.01),显著提高血清TP及ALB含量(P<0.01)。消黄方显著改善肝组织病理和胶原染色,三氯化钆无此显著作用。结论具有清热利湿、活血化瘀功用的消黄方显著干预DMN诱导大鼠肝硬化形成。
Objective To investigate the effect of Xiaohuang Decoction on the progression of chronic cirrhosis induced by dimethylnitrosamine (DMN) in rats. Methods Thirty-four Wistar male rats were randomly divided into normal group (n = 10) and model group (n = 24). The model of liver cirrhosis was made by intraperitoneal injection of DMN for 4 weeks. The model rats were randomly divided into Xiahuang Fang group, gadolinium trichloride group and model control group (7 mice in each group). Xiaoxifang group Continue to model the same time daily Xiaoyan decoction given 2 weeks; gadolinium trichloride group twice weekly gadolinium (7mg / kg) intravenous injection. All rats were sacrificed at 4 weeks. Observe the death of rats, body weight, liver morphology, liver function, liver and spleen weight, liver histological changes. Results DMN model 2 weeks, the rat liver showed significant fibrosis, the formation of cirrhosis of the rat model 4 weeks. Compared with the normal group, the levels of serum ALT, AST, ALP and TBiL increased with the time of modeling Trapezoid increased; while the content of serum total protein (TP) and albumin (Alb) decreased gradually with the progress of model; Compared with the model control group, Xiaohuangfang group decreased serum ALT, AST and ALP activity and TBiL content P <0.05 or P <0.01), significantly increased serum TP and ALB levels (P <0.01). Xiaohuang Decoction significantly improve liver tissue pathology and collagen staining, gadolinium trichloride no such significant role. Conclusion Xiaoyan decoction, which has the functions of clearing away heat, dampness and promoting blood flow and removing blood stasis, significantly interferes with DMN-induced hepatic cirrhosis formation.