论文部分内容阅读
目的探讨骨肉瘤肿瘤细胞胸苷激酶1(TK1)的表达状况及骨肉瘤患者血清TK1的水平,分析其临床病理意义。方法采用免疫组化MaxVision法检测40例骨肉瘤和20例骨巨细胞瘤组织标本中TK1的表达;点印迹免疫酶化学发光法检测20例骨肉瘤患者和20例骨外伤患者血清TK1的水平。结果骨肉瘤肿瘤细胞TK1阳性信号位于胞浆及胞核,高级别骨肉瘤阳性率显著高于低级别骨肉瘤(P<0.05);骨肉瘤肿瘤细胞TK1阳性率亦显著高于骨巨细胞瘤(P<0.05);骨肉瘤患者血清TK1的水平(2.95±1.33)pmol/L(0.97~7.25 pmol/L)高于外伤除外肿瘤患者(0.89±0.51)pmol/L(0.32~2.58 pmol/L),两者差异具有显著性统计学意义(P<0.05)。结论 TK1在骨肉瘤与骨的良性病变的鉴别诊断中具有一定的意义。此外,TK1在骨肉瘤患者血清中可被检测到较高的水平,是一项检测骨肉瘤增殖活性较灵敏的指标。
Objective To investigate the expression of thymidine kinase 1 (TK1) in osteosarcoma tumor cells and the level of serum TK1 in patients with osteosarcoma and to analyze the clinicopathological significance. Methods Immunohistochemical MaxVision method was used to detect the expression of TK1 in 40 osteosarcoma and 20 giant cell tumor of bone. TK1 level was detected by dot blot immunoenzyme chemiluminescence assay in 20 osteosarcoma patients and 20 osteosarcoma patients. Results The positive rate of TK1 in osteosarcoma tumor cells was located in cytoplasm and nucleus, the positive rate of high grade osteosarcoma was significantly higher than that in low grade osteosarcoma (P <0.05). The positive rate of TK1 in osteosarcoma tumor cells was also significantly higher than that of giant cell tumor of bone (2.95 ± 1.33) pmol / L (0.97 ~ 7.25 pmol / L) in patients with osteosarcoma was significantly higher than that in patients with traumatic tumors (0.89 ± 0.51 pmol / L, 0.32-2.58 pmol / L) , The difference between the two was statistically significant (P <0.05). Conclusion TK1 has certain significance in the differential diagnosis of benign lesions of osteosarcoma and bone. In addition, TK1 can be detected in serum of patients with osteosarcoma to a higher level, is a detection of osteosarcoma proliferation activity more sensitive indicators.