Transient receptor potential channel A1 involved in calcitonin gene-related peptide release in neuro

• 作 者: Nobumasa Ushio Yi Dai Shenglan Wang Tetsuo Fukuoka Koichi Noguchi
• 机 构: Department of Anatomy and Neuroscience,Hyogo College of Medicine,Department of Pharmacy,School of Ph
• 出 处: Neural Regeneration Research
• 发表日期: 2013年32期
• 关键词: calcitonin isothiocyanate dorsal allyl sensory CGRP minutes neurogenic cultured

Transient receptor potential channel A1 is one of the important transducers of noxious stimuli in the primary afferents,which may contribute to generation of neurogenic inflammation and hyperalgesia.The present study was designed to investigate if activation of transient receptor potential channel A1 may induce calcitonin gene-related peptide release from the primary afferent neurons.We found that application of allyl isothiocyanate,a transient receptor potential channel A1 activator,caused calcitonin gene-related peptide release from the cultured rat dorsal root ganglion neurons.Knockdown of transient receptor potential channel A1 with an antisense oligodeoxynucleotide prevented calcitonin gene-related peptide release by allyl isothiocyanate application in cultured dorsal root ganglion neurons.Thus,we concluded that transient receptor potential channel A1 activation caused calcitonin gene-related peptide release in sensory neurons.

Transient receptor potential channel A1 is one of the important transducers of noxious stimuli in the primary afferents, which may contribute to generation of neurogenic inflammation and hyperalgesia. The present study was designed to investigate if activation of transient receptor potential channel A1 may induce calcitonin gene- related peptide release from the primary afferent neurons. We found that application of allyl isothiocyanate, a transient receptor potential channel A1 activator, caused calcitonin gene-related peptide release from the cultured rat dorsal root ganglion neurons. Knockdown of transient receptor potential channel A1 with an antisense oligodeoxynucleotide prevented calcitonin gene-related peptide release by allyl isothiocyanate application in cultured dorsal root ganglion neurons.Thus, we concluded that transient receptor potential channel A1 activation caused calcitonin gene-related peptide release in sensory neurons.