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To better understand the interaction between Schistosoma japonicum and its murine host,wecharacterized the immune response of CD4~+ T cells generated during an experimental S.japonicum infectionbased on different key aspects,from gene expression to cell behavior.Mouse oligonucleotide microarrayswere used to compare gene expression profiles of CD4~+ T cells from spleens of mice at 0,3,6 and 13weeks post-infection.Flow cytometry analysis was used to determine type 1 and type 2 cytokine-secretingCD4~+ T cells,to test apoptosis of CD4~+ T cells and to count CD4~+CD25~+ T cells,a kind of regulatorysubpopulation of CD4~+ T cells.The percentage of interleukin-4-producing CD4~+ T cells was found to bemuch higher than that of γ-interferon-producing cells,especially after stimulation with S.japonicum eggantigen,which was consistent with type 1 and type 2 cytokine gene expression in the genechip.Microarraydata also showed that S.japonicum induced the increased expression of Th2 response-related genes,whereassome transcripts related to the Th1 responsive pathway were depressed.Flow cytometry analysis showeda marked increase in the apoptotic CD4~+ T cells from 6 weeks post-infection and in the ratio of CD4~+CD25~+to CD4~+ T cells in infected mice after 13 weeks.We therefore concluded that experimental infection of micewith S.japonicum resulted in a Th2-skewed immune response,which was to a great extent monitored bythe immune regulatory network,including cytokine cross-modulation,cell apoptosis and the subpopulationof regulatory cells.
To better understand the interaction between Schistosoma japonicum and its murine host, wecharacterized the immune response of CD4 ~ + T cells generated during an experimental S. japonicum infection based on different key aspects, from gene expression to cell behavior. Mouse oligonucleotide microarrays used to compare gene expression profiles of CD4 ~ + T cells from spleens of mice at 0,3,6 and 13 weeks post-infection. Flow cytometry analysis was used to determine type 1 and type 2 cytokine-secreting CD4 ~ + T cells, to test apoptosis of CD4 ~ T cells and to count CD4 ~ + CD25 ~ + T cells, a kind of regulator of subpopulation of CD4 ~ + T cells. The percentage of interleukin-4-producing CD4 ~ + T cells was found to be much higher than that of γ-interferon -producing cells, especially after stimulation with S. japonicum eggantigen, which was consistent with type 1 and type 2 cytokine gene expression in the genechip. Microarraydata also showed that S. japonicum induced the increased expression of Th2 response-related genes, whereassome transcripts related to the Th1 responsive pathway were depressed. Flow cytometry analysis showed a marked increase in the apoptotic CD4 ~ + T cells from 6 weeks post-infection and in the ratio of CD4 ~ + CD25 ~ + to CD4 ~ + T cells in infected mice after 13 weeks. We therefore concluded that experimental infection of mice with S.japonicum resulted in a Th2-skewed immune response, which was to a great extent monitored by the immune regulatory network, including cytokine cross-modulation, cell apoptosis and the subpopulationof regulatory cells.