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采用微机电系统(MICROELECTROMECHANICALSYSTEMS,MEMS)技术制备安培型免疫传感器电极系统,并利用A蛋白和硫醇自组装单层膜(SELF-ASSEMBLEDMONOLAYER,SAM)设计传感界面,用于抗体的定向固定,研制了一种新型的安培型免疫传感器.首先采用MEMS技术,在硅片上制备“AU,PT,PT”微型三电极系统和SU-8微型反应池;然后基于自组装技术,先在金电极上制备巯基乙胺自组装单层膜,利用静电吸附作用在巯基乙胺单层膜上组装A蛋白,再利用A蛋白对抗体的定向吸附作用实现抗体在金电极表面的定向固定.该传感器的研制以低成本、微型化、高度集成,以及实现“片上系统”生物传感器为目标.采用循环伏安法和计时电流法研究传感器的响应特性,与采用块体电极的传统传感器以及采用在电极上直接吸附A蛋白的抗体固定方法相比,该传感器体积小,与CMOS集成电路工艺兼容,对人免疫球蛋白(HIGG)的测定响应快(20S),线性范围宽(50~400ΜG/L),检出限低(10ΜG/L),并且易于实现传感器的阵列化和实时多参数检测.
Ampere-type immunosensor electrode system was prepared by micro-electromechanical system (MEMS) technology. The sensing interface was designed by SELF-ASSEMBLEDMONOLAYER (SAM) A new type of amperometric immunosensor was developed.At first, the micro-three-electrode system of AU, PT, PT and SU-8 micro-reactor were fabricated on silicon by MEMS technology. Then, based on the self-assembly technique, Preparation of self-assembled monolayers of mercaptoethylamine, the use of electrostatic adsorption on the mercaptoethylamine monolayers assembled protein A, and then use the protein A of the antibody directed adsorption of antibodies on the surface of the gold electrode orientation fixed. The development of the sensor The goal is to achieve low cost, miniaturization, highly integrated and “system-on-chip” biosensors.Cyclic voltammetry and chronoamperometry were used to study the response characteristics of the sensor, compared with traditional sensors using bulk electrodes, Compared with the antibody immobilization method for adsorbing protein A, the sensor is small in volume and compatible with the CMOS integrated circuit technology. The determination of human immunoglobulin (HIGG) Should be fast (20S), wide linear range (50 ~ 400ΜG / L), low detection limits (10ΜG / L), and arrays and easy to implement real-time multi-parameter detection sensor.