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【目的】对水稻lrd表达的光温诱导反应进行研究,明确水稻lrd表达光诱导波段和温度的抑制或促进作用。【方法】利用自有的6个水稻lrd突变体,用LED单色光源诱激和人工气候箱中不同温度梯度处理,观察类病斑的发生情况和统计病斑个数,比较诱导的效果。【结果】lrd31类病斑表达受紫外光诱导,lrd36的表达受黄光诱导,lrd37和lrd35的表达受蓝光诱导,而lrd32和lrd40在所有处理的光源下均表达类病斑;lrd32、lrd36和lrd37等3种温度下都正常产生病斑,低温明显促进病斑的发生。lrd35和lrd40在高温下不表达,在中温下可正常表达,低温下也表现促进作用,但lrd31只有在中温下正常表达,低温和高温抑制病斑出现。【结论】不同的lrd基因型所需的光诱导反应波段是不相同的。温度是抑制或促进lrd表达的另一个重要环境因子,一般是较低的温度促进lrd表达,高温抑制lrd表达。
【Objective】 The objective of this study was to study the light and temperature-induced responses of lrd expression in rice and to determine the inhibition or promotion of light-induced band and temperature in rice lrd expression. 【Method】 The 6 ldy mutants of rice were treated with the monochromatic light source of LED and different temperature gradients in the artificial climate chamber to observe the occurrence of the lesion and count the number of the lesion and to compare the induced effects. 【Result】 The expression of lrd31 was induced by ultraviolet light. The expression of lrd36 was induced by yellow light. The expression of lrd37 and lrd35 was induced by blue light, while lrd32 and lrd40 expressed lesions like lrd32, lrd36 and lrd37 and other 3 kinds of temperatures are normal lesions, low temperature significantly promote the occurrence of lesions. lrd35 and lrd40 were not expressed at high temperature, but were expressed normally at moderate temperature and also promoted at low temperature. However, lrd31 was only expressed at moderate temperature and inhibited at low temperature and high temperature. 【Conclusion】 The light-induced reaction bands required for different lrd genotypes are different. Temperature is another important environmental factor that inhibits or promotes lrd expression. Generally, lower temperature promotes lrd expression and high temperature inhibits lrd expression.