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为了检测胰腺癌患者树突状细胞(dendritic cells,DC)诱导I型调节性T细胞(typeⅠregulatory T cells,Tr1)的特征、功能及其临床意义。采用外周血单核细胞来源的未成熟DC,诱导同种异体初始T细胞分化为Tr1,ELISA、流式细胞仪检测Tr1细胞因子表达水平。用混合淋巴细胞反应(mixed lymphocyte reaction,MLR)检测Tr1的免疫抑制功能。结果:经胰腺癌患者DC诱导分化的Tr1分泌IL-10(P<0.05)和TGF-β(P<0.01)水平高于正常对照组。IL-10胞内染色结果也表明,分泌IL-10的Tr1在胰腺癌组明显增加(P<0.01)。胰腺癌患者DC诱导的Tr1抑制MLR增殖的能力也明显增强(P<0.01)。胰腺癌患者DC诱导同种异体Tr1分化的能力明显增强,提示过度Tr1活化可能与胰腺癌的病理形成有关。
To investigate the characteristics, functions and clinical significance of dendritic cells (DC) -induced type I regulatory T cells (TrI) in patients with pancreatic cancer. Immature DCs derived from peripheral blood mononuclear cells were used to induce allogeneic T cells to differentiate into Tr1. The expression of Tr1 cytokine was detected by flow cytometry. The immunosuppressive function of Tr1 was tested by mixed lymphocyte reaction (MLR). Results: The levels of IL-10 (P <0.05) and TGF-β (P <0.01) secreted by Tr1 induced by DC in pancreatic cancer patients were higher than those in normal controls. IL-10 intracellular staining also showed that Tr1 secretion of IL-10 in pancreatic cancer group was significantly increased (P <0.01). The ability of Tr1 induced by DC induced by pancreatic cancer to inhibit the proliferation of MLR was also significantly enhanced (P <0.01). The ability of DC induced allogenic Tr1 differentiation in pancreatic cancer patients was significantly enhanced, suggesting that excessive Tr1 activation may be related to the pathological formation of pancreatic cancer.