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目的研究海人酸(KA)对大鼠海马中生长相关蛋白-43(GAP-43)和神经细胞粘附分子(NCAM)表达的影响,以及托吡酯(TPM)对其的干预作用。方法将48只大鼠随机分成生理盐水(NS)组、4mg KA组、10mg KA组和10mg KA+TPM组(n=12),建立KA诱导颞叶癫大鼠模型和TPM干预模型,观察大鼠行为学改变,并通过RT-PCR和Western Blot的方法测定各组大鼠海马中GAP-43及NCAM的mRNA和蛋白表达水平。结果大鼠建模成功;4mg KA组、10mg KA组大鼠GAP-43及NCAM的mRNA和蛋白表达水平明显高于NS组(P<0.01),且10mg KA组高于4mg KA组(P<0.01);10mg KA+TPM组大鼠的GAP-43和NCAM表达明显低于10mg KA组(P<0.01)。结论KA能够诱导致大鼠海马GAP-43和NCAM表达上调,且与KA剂量有关,而TPM能够抑制KA诱导的GAP-43和NCAM的表达上调。
Objective To investigate the effect of kainate on the expression of GAP-43 and NCAM in hippocampus of rats and the effect of topiramate (TPM) on it. Methods 48 rats were randomly divided into NS group, 4 mg KA group, 10 mg KA group and 10 mg KA + TPM group (n = 12). The model of temporal lobe epilepsy induced by KA and the intervention model of TPM were established. The behavioral changes of rats were observed. The mRNA and protein expressions of GAP-43 and NCAM in hippocampus were determined by RT-PCR and Western Blot. Results The rat model was successfully established. The mRNA and protein expressions of GAP-43 and NCAM in 4 mg KA group and 10 mg KA group were significantly higher than those in NS group (P <0.01), and were higher in 10 mg KA group than in 4 mg KA group (P < 0.01). The expression of GAP-43 and NCAM in 10 mg KA + TPM group was significantly lower than that in 10 mg KA group (P <0.01). Conclusion KA can induce the up-regulation of GAP-43 and NCAM in the hippocampus of rats and is related to the dosage of KA. TPM can inhibit the up-regulation of GAP-43 and NCAM induced by KA.