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研究了小麦Ｄ~２型ＣＭＳ系ｍｓＤ~２－ＣＡ８０５７与保持系ＣＡ８０５７花粉发育的细胞形态学特征及花粉粒“单核－双核期”、“三核期”的花药、雌蕊、旗叶和授粉２０天左右的灌浆期种子胚乳的过氧化物酶同工酶、酯酶同工酶，主要研究结果揭示：Ｄ~２型不育系花粉败育起始于“单核－双核期”，表现为败育期长和败育方式多样化；“三核期”花药的同工酶有明显差异，主要表现为不育系酯酶比保持系少３条酶带且酶的总活性比保持系的弱、过氧化物酶的总活性比保持系弱很多且少３条酶带。上述差异可能是Ｄ~２型细胞质不育基因对核基因的调控表达所致，而这种调控作用可能是导致雄性不育形成的原因之一；这些特征也表明Ｄ~２型不同于Ｔ型也不同于Ｋ型，是个新的不育类型。
The morphological characteristics of pollen development in pollen grains of “D-2 CMS” msD ~ 2-CA8057 and maintainer CA8057 were studied, and anther, pistil, flag leaf and pollination The main research results of the peroxidase isoenzyme and esterase isozyme in the seed endosperm during the filling stage of about 20 days revealed that the pollen abortion in the D ~ 2 type sterile line originated in the “mononuclear - dinuclear phase” The length of abortion period and the abortion pattern were diversified. The isozymes of anthers of the “trinuclear stage” were significantly different, mainly showing less enzyme bands than the maintainer lines and the total activity ratio The total activity of peroxidase is much weaker than that of the maintainer and less 3 bands. These differences may be due to the regulation of nuclear gene expression by D ~ 2 cytoplasmic male sterility genes, which may be one of the reasons leading to the formation of male sterility. These characteristics also indicate that D ~ 2 is different from T Also different from K type, is a new type of infertility.