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目的建立同时测定灯银脑通胶囊中灯盏乙素、白果内酯、银杏内酯A、银杏内酯B、人参皂苷Rg1和三七皂苷R1等6种有效成分含量的HPLC-MS/MS方法。方法选用Phenomenex Luna C_(18)(4.6 mm×150 mm,5μm)色谱柱,0.2%甲酸甲醇溶液(A)、0.2%甲酸水溶液(B)梯度洗脱方式洗脱,柱温为30℃,流速为0.3 mL·min~(-1),分析时间10.0 min;质谱条件选用ESI源-MRM模式,正离子模式检测。建立的方法对3批灯银脑通胶囊进行了测定。结果灯盏乙素、白果内酯、银杏内酯A、银杏内酯B、人参皂苷Rg1和三七皂苷R_16种有效成分在测定浓度范围内均具有良好的线性关系,r均不小于0.999 0,提取回收率均在94.8%~108.5%之间。结论本法准确可靠、结果稳定、专属性强、重现性良好,为灯银脑通胶囊的质量控制提供了很好的依据。
OBJECTIVE To establish a HPLC-MS / MS method for the simultaneous determination of 6 active ingredients in Deng Yin Nao Tong Capsules, such as scutellarin, bilobalide, ginkgolide A, ginkgolide B, ginsenoside Rg1 and notoginsenoside R1. Methods The column was eluted with a gradient of Phenomenex Luna C_ (18) (4.6 mm × 150 mm, 5 μm), 0.2% formic acid in methanol (A) and 0.2% formic acid in water 0.3 mL · min -1, and the analysis time was 10.0 min. The mass spectrometry conditions were ESI source-MRM mode and positive ion mode. The established method of 3 batches of lamp silver brain capsule was determined. Results Scutellarin, bilobalide, ginkgolide A, ginkgolide B, ginsenoside Rg1 and notoginsenoside R_16 active ingredients in the determination of the concentration range has a good linear relationship, r are not less than 0.999 0, extraction The recoveries ranged from 94.8% to 108.5%. Conclusion This method is accurate, reliable, stable, specific and reproducible. It provides a good basis for the quality control of Deng Yin Nao Tong Capsule.