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作者对暴发性肝炎(FH)和其他肝病患者进行了外周血单核细胞(PBMC)白三烯B4(LTB4)产生率的检测。研究对象包括17例FH患者,9例急性肝炎(AH)患者,6例慢性肝炎及5例伴肝细胞癌的肝硬变患者。对照组为10例健康志愿者。 病人入院后即刻采集血液标本,其后的标本可在早晨血浆交换前采集。在钙离子载体剌激下,PBMC培养某中的LTB4经HPLC分析后用放射免疫法检测。细胞培养最初15分钟期间,未检测到有LTB4的自发
The authors tested the incidence of peripheral blood mononuclear cells (PBMC) leukotriene B4 (LTB4) in patients with fulminant hepatitis (FH) and other liver diseases. Participants included 17 patients with FH, 9 patients with acute hepatitis (AH), 6 patients with chronic hepatitis and 5 patients with cirrhosis with hepatocellular carcinoma. The control group was 10 healthy volunteers. Blood samples were collected immediately after admission to the patient, and subsequent samples were collected before morning plasma exchange. Under the stimulation of calcium ionophore, LTB4 in culture of PBMC was detected by radioimmunoassay after HPLC analysis. During the first 15 minutes of cell culture, no spontaneous LTB4 was detected