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我们在研究１２５Ｉ标记胰岛素、超氧化物歧化酶（ＳＯＤ）和牛血清白蛋白（ＢＳＡ）等一些激素、蛋白质类物质的同时，对４种测定方法进行了实验比对，提出采用简单、方便、省时的ＴＣＡ沉淀法测定标记率较为适宜。标记率是评价一个标记方法好坏和标记条件选择是否恰当的重要指标［１］，通过它还能较为简便地计算出被标记物的比活度［２］。通常，１２５Ｉ标记物的标记率测定采用淋洗分离时标记产品峰计数率与诸峰计数率之和的比值［３］或标记产品峰的计数率与校正后１２５Ｉ投料量的比值［４］来确定，有时也采用对标记液进行层析、电泳、三氯醋酸（ＴＣＡ）沉淀等方法来测定。
In the study of 125I labeled insulin, superoxide dismutase (SOD) and bovine serum albumin (BSA) and some other hormones, protein substances at the same time, four kinds of determination methods were experimentally aligned, and proposed a simple, convenient, provincial When TCA precipitation method to determine the labeling rate is more appropriate. The labeling rate is an important index to evaluate whether a labeling method is good or not and the selection of labeling conditions is appropriate [1]. It is also easier to calculate the specific activity of labeled substances [2]. In general, the labeling rate of 125I labeling is determined by the ratio of the peak count rate to the sum of peak count rates [3] or the ratio of the count peak rate of the labeled product to the corrected 125I feed volume [4] To determine, and sometimes also used on the labeling solution chromatography, electrophoresis, trichloroacetic acid (TCA) precipitation and other methods to determine.