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目的 动态检测SARS患者血浆特异性IgM和IgG以及SARS冠状病毒(SARS CoV)的变化规律。方法 用酶联吸附免疫法对 2 5例 14 5份SARS临床诊断患者血浆特异性IgM和IgG进行定性检测 ,用RT PCR对其中 114份血浆进行SARS CoV定性检测。结果 IgM和IgG抗体的阳性样本检出率分别为 4 9 0 % (71/14 5 )和 5 4 5 % (79/14 5 ) ,阳性患者检出率均为 84 0 % (2 1/2 5 ) ,两种抗体大多在发病第 2～4周产生 ,前 5周检出率相近 ,均呈上升趋势 ,以后IgM抗体检出率开始下降 ,IgG抗体检出率则继续上升。血浆SARS CoV阳性样本检出率为 15 8% (18/114 ) ,阳性患者检出率为 4 0 0 % (10 /2 5 ) ,多为发病后 4周内采集的样本 ,抗体检测阴性或初次阳性。结论 血浆特异性抗体以及SARS CoV检测可作为SARS的确诊依据 ;抗体产生后大多数病人血浆病毒很快转阴 ,但有个别病人在抗体产生2～ 3周后仍能在血浆中检测到病毒序列
Objective To dynamically detect the changes of plasma specific IgM and IgG in SARS patients and the SARS CoV. Methods Serum specific IgM and IgG in 25 cases of clinical diagnosis of SARS were detected by enzyme-linked immunosorbent assay (ELISA), and 114 plasma samples were qualitatively detected by RT-PCR. Results The positive rates of IgM and IgG were 49.0% (71/14 5) and 54.5% (79/14 5), respectively. The positive rate of positive samples was 84 0% (2 1/2 5). Most of the two antibodies were produced in the second to fourth weeks after onset. The detection rates of the two antibodies were similar in the first five weeks, and then the detection rate of IgM antibody began to decline. The detection rate of IgG antibody continued to rise. The detection rate of CoV positive samples in plasma SARS was 15 8% (18/114), the positive rate was 40% (10/2 5), mostly the samples collected within 4 weeks after the onset of disease. The antibody was negative or Positive for the first time. Conclusions Plasma-specific antibodies and SARS-CoV detection can be used as the basis for the diagnosis of SARS. Most of the patient’s plasma virus turns negative soon after the antibody is produced. However, some patients still can detect the virus sequence in plasma 2 to 3 weeks after antibody production