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目的 :探索Fenton反应 (Fe2 ++H2 O2 →Fe3++·OH +OH-)氧化损伤DNA的实验条件。方法∶气相色谱/质谱分析法探索Fenton反应对DNA中 8-羟基鸟嘌呤含量的影响 ,观察经不同浓度的H2 O2 和Fe2 +及不同时间处理后DNA样品中 8-羟基鸟嘌呤含量的改变。结果∶Fenton反应显著升高DNA中 8-羟基鸟嘌呤含量。 8-羟基鸟嘌呤含量随H2 O2 浓度的改变而呈三相变化∶H2 O2 浓度在 0 .0 8～ 5mmol/L范围时 ,8-羟基鸟嘌呤含量随H2 O2 浓度的升高而升高 ;H2 O2 浓度在 5～ 2 0mmol/L范围时 ,其含量随H2 O2 浓度的升高而减少 ;H2 O2 浓度在 2 0～ 80mmol/L范围时 ,8-羟基鸟嘌呤含量维持在一个较对照组高的水平 ,而不随H2 O2 浓度的改变而发生明显的改变。增加Fe2 +浓度或延长反应时间均可提高DNA中 8-羟基鸟嘌呤的含量。结论∶建立了一个Fenton反应氧化损伤DNA的模型
Objective: To explore the experimental conditions of oxidative DNA damage induced by Fenton reaction (Fe2 ++ H2 O2 → Fe3 ++ · OH + OH-). Methods: The effects of Fenton reaction on the content of 8-hydroxy guanine in DNA were investigated by gas chromatography / mass spectrometry (GC / MS). The changes of 8-hydroxyguanosine content in DNA samples treated with different concentrations of H2O2 and Fe2 + Results: Fenton reaction significantly increased DNA 8-hydroxy guanine content. The content of 8-oxoguanine showed a three-phase change with the change of H2O2 concentration: the content of 8-oxoguanine increased with the increase of H2O2 concentration when the concentration of H2O2 was in the range of 0.08-5mmol / L; H2O2 concentration in the range of 5 ~ 20mmol / L, its content decreases with the increase of H2O2 concentration; H2O2 concentration in the range of 20 ~ 80mmol / L, 8-hydroxy guanine content was maintained at a control group High level without significant changes in the concentration of H2O2. Increasing the concentration of Fe2 + or prolonging the reaction time can increase the content of 8-hydroxyguanine in the DNA. Conclusion: A DNA oxidative damage model was established by Fenton reaction