Cmparison of cleavage activity in vitro between two ribozymes targeted against different sites of PD

• 作 者: 陆翠华 陈岳祥 等
• 机 构: DepartmentofGastroenterology,DepartmentofGastroenterology
• 出 处: 中国人民解放军军医大学学报：英文版
• 发表日期: 2002年1期
• 关键词: 核酶 PGDF受体 Β亚单位 体外转录 体外分裂 ribozyme i PDGF receptor β subunit transcription in v
• 基金项目: Supported by the National Natural Science Foundation of China (No. 39870303)

<正>Objective: To compare the cleavage activity of ribozymes directed against 2 sites of PDGF receptorP subunit cDNA gene in vitro. Methods: The 608 bp fragment of PDGF receptor β subunit cDNA was clonedinto T-vector under the control of T7 promoter,