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目的建立除草剂2,4-D丁酯的气相色谱一质谱(GC/MS)检测方法,用于临床中毒患者的检测。方法以乙酸乙酯提取血液中2,4D丁酯,采用GC/MS检测。定性采用全扫描法(SCAN),定量采用选择离子法(SIM),离子选择m/z 175、185、276。结果2,4-D丁酯在10～1500 ng/ml成线性,方程为Y=10.565X-1203.5,R=0.9941,标加1000 ng/ml浓度的2,4-D丁酯于血液,平均回收率为94.6%,RSD=7.1%;2,4-D丁酯最低检出浓度为3 ng/ml(SIM),信噪比S/N=3;日内精密度RSD为4.1%,日间精密度RSD为5.8%。结论所建方法灵敏、快速、线性范围好,可用于2,4-D丁酯中毒患者的检测。
Objective To establish a gas chromatography-mass spectrometry (GC / MS) method for the determination of 2,4-D butylate, which is used in the detection of clinical poisoning patients. Methods Blood 2,4D butyl ester was extracted with ethyl acetate and detected by GC / MS. Qualitatively, SCAN was used, quantitatively selected ion mode (SIM) and ion selective m / z 175,185,276. Results Linearity of 2,4-D butyl ester was between 10 and 1500 ng / ml. The equation was Y = 10.565X-1203.5, R = 0.9941. The average concentration of 2,4-D butyl ester in blood was 1000 ng / The minimum detectable concentration of 2,4-D butyl was 3 ng / ml (SIM) and the S / N ratio was S / N = 3. The intraday RSD was 4.1% RSD precision of 5.8%. Conclusion The method is sensitive, rapid and has good linearity. It can be used in the detection of 2,4-D butyl poisoning.