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目的研究异丙酚在小鼠睡眠剥夺模型学习记忆和海马区神经炎症中的作用。方法将小鼠随机分为对照组、睡眠剥夺组、异丙酚预处理组。采用多平台水环境法建立睡眠剥夺小鼠模型,异丙酚预处理组小鼠造模前尾静脉注射25 mg/kg异丙酚。采用Morris水迷宫法检测小鼠的学习记忆能力,并检测小鼠脑海马IL-1β、IL-6和小胶质细胞TLR4、My D88、TRIF、Iba-1的表达。结果睡眠剥夺组小鼠实验前、后体重与对照组、异丙酚预处理组小鼠体重比较差异无统计学意义,而进食量增加;睡眠剥夺组小鼠潜伏期更长,并且撤去平台后于目标象限中游泳时间所占百分比更小,而异丙酚预处理组则缩短潜伏期,提高目标象限游泳时间所占百分比;睡眠剥夺小鼠脑海马中炎症因子IL-1β、IL-6及小胶质细胞中Iba-1、TLR4、My D88、TRIF表达水平显著高于异丙酚预处理组与对照组。结论异丙酚预处理具有改善睡眠剥夺小鼠学习记忆障碍并减轻神经炎症的作用。
Objective To investigate the effect of propofol on learning and memory impairment and neuroinflammation in hippocampus in mice with sleep deprivation. Methods The mice were randomly divided into control group, sleep deprivation group and propofol pretreatment group. A sleep-deprived mouse model was established by a multi-platform water environment method. Propofol was injected into the caudal vein of propofol-treated mice at a dose of 25 mg / kg. Morris water maze test was used to detect the learning and memory abilities of mice and the expressions of IL-1β, IL-6 and microglia TLR4, My D88, TRIF and Iba-1 in the hippocampus were detected. Results There was no significant difference in body weight between the sleep deprived mice before and after the experiment and the control group and the propofol pretreatment group, but the food intake increased. The mice in the sleep deprivation group had longer incubation period, The percentage of swimming time in the target quadrant was smaller, while the propofol preconditioning group shortened the incubation period and increased the percentage of target quadrant swimming time; sleep deprivation mouse brain hippocampus inflammatory cytokines IL-1β, IL-6 and small glue The expression of Iba-1, TLR4, My D88 and TRIF in stromal cells was significantly higher than that in propofol pretreatment group and control group. Conclusion Propofol preconditioning can improve sleep deprivation mice learning and memory impairment and reduce the role of neuroinflammation.