论文部分内容阅读
肽库是由一大群被用于筛选的寻找新的药理先导作用的寡聚肽组成的。它们的合成是藉固相以组合方式来实现的 ,即反应成分之一是氨基酸或肽与树脂结合的混合物 ,或者是氨基酸混合物的溶液。实际上 ,在生成的肽库中各种肽不是以等分子的状态存在的。作者说明了在肽库设计中统计学手段的重要性。指出一珠一肽的肽库形式在多大程度上符合统计学的标准 ,并讨论了使库中存在的不同肽的相对浓度范围极小化所需树脂珠的数量。此范围对于正确解释生物测定的结果极为重要
The peptide pool consists of a large group of oligopeptides that are used for screening to find new pharmacological leads. Their synthesis is achieved by a combination of solid phases, that is, one of the reactive components is a mixture of amino acids or peptides and a resin, or a mixture of amino acids. In fact, various peptides are not present in the isomolecular state in the generated peptide library. The authors demonstrated the importance of statistical methods in the design of peptide libraries. The extent to which the peptide library forms of the peptides meet the statistical criteria is stated, and the number of resin beads required to minimize the relative concentration range of the different peptides present in the library is discussed. This range is extremely important for correctly interpreting the results of biometrics