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目的:初步研究环维黄杨星D(Cvb-D)对小鼠心肌肥厚的保护作用。方法:皮下注射异丙肾上腺素(Iso)1mg·kg~(-1),bid,连续14d,制备小鼠心肌肥厚模型,各治疗组分别给予环维黄杨星D(6,9,12mg·kg~(-1))和阳性对照卡托普利(100mg·kg~(-1))灌胃给药,末次给药24h后处死小鼠,测量小鼠心脏质量指数(全心质量/体质量)和左心室质量指数(左心室质量/体质量),并观察心肌组织病理形态学改变,以评价Cvb-D对小鼠心肌肥厚的保护作用。结果:与空白对照组比较,Iso模型组小鼠心脏重量指数明显升高,病理切片可见心肌肥厚改变。与Iso组相比,Cvb-D高剂量组能明显降低小鼠心脏质量指数和左心室质量指数(P<0.05或0.01),病理切片可见损害较模型组减轻。结论:环维黄杨星D对Iso诱导的小鼠心肌肥厚具有一定的保护作用,其机制有待进一步研究阐明。
Objective: To study the protective effect of Cyclovirobuxine D (Cvb-D) on myocardial hypertrophy in mice. METHODS: Isoproterenol (Iso) 1 mg·kg -1 (bid) was injected subcutaneously for 14 days to establish a mouse cardiac hypertrophy model. Cyclovirobuxine D (6,9,12 mg·kg) was administered to each treatment group. ~(-1)) and positive control captopril (100 mg·kg -1) were intragastrically administered and mice were sacrificed 24 h after the last administration. Measure the heart quality index (whole heart quality/body weight) in the mice. ) and left ventricular mass index (left ventricular mass / body mass), and observe the pathological changes of myocardial tissue, in order to evaluate the protective effect of Cvb-D on cardiac hypertrophy in mice. RESULTS: Compared with the blank control group, the heart weight index of the Iso model group significantly increased, and the pathological section showed changes in cardiac hypertrophy. Compared with the Iso group, the Cvb-D high-dose group significantly reduced the heart quality index and the left ventricular mass index (P<0.05 or 0.01), and the pathological slice showed less damage than the model group. Conclusion: Cyclovirobuxine D has certain protective effect on Iso-induced myocardial hypertrophy in mice. The mechanism remains to be further elucidated.