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目的：探讨细胞因子对小鼠α２（Ｉ）型前胶原基因上游启动子活性的影响。方法：以小鼠α２（Ｉ）型前胶原基因启动子（－２ｋｂ～＋５４ｂｐ）为靶序列，将它与氯霉素乙酰基转移酶（ＣＡＴ）报告基因组成的重组体ｐＡＺ１００９用脂质体法转染至胶原产生细胞ＮＩＨ／３Ｔ３细胞，讨论ＩＦＮα、ＩＦＮγ、ＴＮＦα对小鼠α２（Ｉ）型前胶原基因上游启动子活性的影响。结果：ＴＮＦα抑制小鼠α２（Ｉ）型前胶原基因启动子活性，ＴＮＦα分别与ＩＦＮγ、ＩＦＮα联合应用能加强这种抑制作用。结论：ＴＮＦα分别与ＩＦＮγ、ＩＦＮα联合应用能加强抑制作用，这可能与ＩＦＮγ、ＩＦＮα诱导ＴＮＦα受体表达，从而加强ＴＮＦα抑制作用有关，而ＩＦＮγ抑制胶原蛋白合成作用与该序列无直接相关。
Objective: To investigate the effect of cytokines on the promoter activity of mouse α2 (Ⅰ) procollagen gene upstream. Methods: Recombinant pAZ1009, which was composed of a reporter gene of chloramphenicol acetyltransferase (CAT) and the promoter of mouse α2 (I) procollagen gene (-2kb ~ + 54bp) The effects of IFNα, IFNγ and TNFα on the promoter activity of mouse α2 (I) procollagen gene were investigated. Results: TNFα inhibited the promoter activity of mouse α2 (I) procollagen gene promoter. The combination of TNFα and IFNγ and IFNα could enhance the inhibitory effect. CONCLUSION: The combination of TNFα and IFNγ and IFNα can enhance the inhibitory effect, which may be related to IFNγ and IFNα inducing the expression of TNFα receptor, thereby enhancing the inhibitory effect of TNFα. However, IFNγ inhibits collagen synthesis without direct correlation with this sequence.