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目的:探讨青藤碱对大鼠原位肝移植缺血再灌注损伤的保护作用。方法:采用Kamada’s“二袖套法”建立SD→SD大鼠原位肝移植模型,随机分为假手术组、对照组、低剂量(40mg/kg)和高剂量(80mg/kg)青藤碱组,分别观察移植术后1周存活率,检测术后2、6、12、24h血清ALT,TUNEL法检测肝细胞凋亡指数(AI),RT-PCR检测肝脏组织中的TNF-α、IL-1β mRNA的含量,并观察移植肝脏病理学改变。结果:与对照组比较,青藤碱低剂量、高剂量组术后1周存活率显著提高(75%、75%vs12.5%,P<0.01);在各个时间点,青藤碱治疗组的ALT水平明显低于对照组(P<0.01),肝组织中的TNF-α、IL-1β mRNA表达与对照组相比均显著降低(P<0.01),肝脏病理学形态也明显改善。结论:青藤碱可以通过抑制TNF-α、IL-1β等炎症性细胞因子合成,抑制肝细胞凋亡,减轻肝细胞及肝窦内皮细胞的损伤,达到保护肝移植缺血再灌注损伤的效果。
Objective: To investigate the protective effect of sinomenine on ischemia-reperfusion injury in orthotopic liver transplantation in rats. METHODS: SD-SD rat orthotopic liver transplantation models were established using Kamada’s two-cuff technique and randomly divided into sham operation group, control group, low dose (40mg/kg) and high dose (80mg/kg) In the rattanine group, the survival rate at 1 week after transplantation was observed. Serum ALT was detected at 2, 6, 12, and 24 hours after operation. Hepatocyte apoptosis index (AI) was measured by TUNEL method. RT-PCR was used to detect TNF-α in liver tissue. , IL-1β mRNA content, and observe the pathological changes of transplanted liver. RESULTS: Compared with the control group, the survival rate of the low-dose and high-dose sinomenine groups was significantly increased after 1 week (75%, 75% vs 12.5%, P<0.01); at each time point, the sinomenine-treated group The ALT level was significantly lower in the control group (P<0.01). The expression of TNF-α and IL-1β mRNA in the liver tissue was significantly lower than that in the control group (P<0.01), and the pathological morphology of the liver was also significantly improved. Conclusion: Sinomenine can inhibit the synthesis of inflammatory cytokines such as TNF-α and IL-1β, inhibit the apoptosis of hepatocytes, and reduce the damage of hepatocytes and sinusoidal endothelial cells, so as to protect the effect of liver transplantation ischemia-reperfusion injury. .