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本文主要对三米单粒种子DNA提取方法及在玉米自交系鉴定中的应用进行了研究，结果表明该方法提取适度快。提取的DNA分子量大．没有降解，质量好，完全可以满足RAPD分析的需要，这就为RAPD在玉米种质及纯度签定上的应用提供了技术上的保证．有利于该项技术的普及和推广。
In this paper, three single grain DNA extraction methods and identification of maize inbred lines were studied, the results show that the method of extracting moderately fast. DNA extracted large molecular weight. No degradation, good quality and fully meet the needs of RAPD analysis, which provides a technical guarantee for the application of RAPD in the corn germplasm and purity. Is conducive to the popularization and promotion of the technology.