论文部分内容阅读
目的 :探讨中药蟾酥灵对HL -60 /ADR耐药细胞在抑制生长、诱导凋亡方面的作用和影响。方法 :①MTT比色法观察对细胞的抑制 ;②流式细胞仪检测细胞凋亡率。结果 :①蟾酥灵抑制HL -60 /ADR耐药细胞的生长 ,药物浓度与HL -60 /ADR细胞生长抑制率之间呈指数关系 ;②随着药物浓度的增加 ,HL -60 /ADR细胞凋亡率也增加。结论 :蟾酥灵可抑制HL -60 /ADR细胞生长 ,促进细胞凋亡呈剂量依赖性
Objective: To investigate the effect and mechanism of Qichangling on HL-60/ADR-resistant cells in inhibiting growth and inducing apoptosis. Methods : 1 MTT colorimetric method was used to observe the inhibition of the cells. 2 Flow cytometry was used to detect the apoptosis rate. RESULTS: 1Wushanling inhibited the growth of HL-60/ADR resistant cells, and the relationship between the drug concentration and the growth inhibition rate of HL-60/ADR cells was exponential; 2 With the increase of drug concentration, HL-60/ADR cells withered The death rate also increased. Conclusion :Wusangling can inhibit the growth of HL-60/ADR cells and promote apoptosis in a dose-dependent manner