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以D,L-丝氨酸为底物,采用色氨酸酶将L-丝氨酸转化为L-色氨酸,并进一步分离纯化拆分得到D-丝氨酸。该文对色氨酸酶酶法拆分条件进行了响应面优化,酶促反应最佳条件为:温度45℃,pH=8.0,反应时间18 h,底物D,L-丝氨酸质量浓度30 g/L,色氨酸酶用量为6 g/L,经过两次转化,L-丝氨酸的总转化率可达95.4%。酶促反应液经NKA-Ⅱ型大孔吸附树脂与001×7强酸性阳离子交换树脂纯化,重结晶后得到D-丝氨酸,化学纯度99.4%,α2D0=-15.3°(ρ=0.1 kg/L,2 mol/L HCl),总回收率为66.6%。
Using D, L-serine as substrate, tryptophanase is used to convert L-serine to L-tryptophan, and further separation and purification to obtain D-serine. In this paper, the enzymatic surface optimization of tryptophanase enzymatic resolution conditions was optimized. The optimal reaction conditions were: temperature 45 ℃, pH = 8.0, reaction time 18 h, substrate D, L-Serine concentration 30 g / L, tryptophanase dosage of 6 g / L, after two transformations, the total conversion of L-serine up to 95.4%. The enzymatic reaction solution was purified by NKA-Ⅱ macroporous adsorption resin and 001 × 7 strong acid cation exchange resin to obtain D-serine after recrystallization, the chemical purity was 99.4%, α2D0 = -15.3 ° (ρ = 0.1 kg / L, 2 mol / L HCl), the total recovery was 66.6%.