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目的:观察茶多酚(tea polyphenols,TP)在不同浓度和不同作用时间下,对由血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)所致的血管内皮细胞分泌内皮素(endothelin,ET)功能的影响,探讨TP对血管内皮细胞的保护作用。方法:将培养的血管内皮细胞分为4个组:空白对照组、AngⅡ(10-7mol/L)组、高浓度(50 mg/L)TP+AngⅡ组、低浓度(25 mg/L)TP+AngⅡ组,采用放射免疫法测定AngⅡ及TP作用前及作用后0.5、6、24 h各组细胞培养上清中的ET含量。结果:(1)AngⅡ组培养上清的ET含量显著高于对照组(P<0.01)。(2)高浓度TP在作用6 h和24 h后,ET含量较AngⅡ组显著下降(P<0.01);而低浓度TP在各作用时间点均较高浓度TP组及AngⅡ显著下降(P<0.01)。结论:TP对AngⅡ所致血管内皮细胞分泌ET的功能具有抑制作用,提示TP对血管内皮细胞具有保护作用;且低浓度TP的保护作用要强于高浓度TP。
Objective: To observe the effect of tea polyphenols (TP) on the function of endothelin (ET) secreted by vascular endothelial cells induced by angiotensin Ⅱ (AngⅡ) at different concentrations and different time periods, To investigate the protective effect of TP on vascular endothelial cells. Methods: The cultured vascular endothelial cells were divided into four groups: blank control group, Ang Ⅱ (10-7mol / L) group, high concentration (50mg / L) TP + AngⅡ group, low concentration + AngⅡgroup. The contents of ET in the cell culture supernatants of each group before and at 0.5, 6 and 24 h after Ang Ⅱ and TP treatment were measured by radioimmunoassay. Results: (1) The content of ET in culture supernatant of Ang Ⅱ group was significantly higher than that of control group (P <0.01). (2) After treated with high concentration of TP for 6 h and 24 h, the content of ET decreased significantly (P <0.01), while the concentration of low concentration of TP increased significantly at each time point (P < 0.01). CONCLUSION: TP inhibits the function of endothelial cells secreting ET induced by angiotensin Ⅱ, suggesting that TP has a protective effect on vascular endothelial cells. The protective effect of TP at a lower concentration is stronger than that of high concentration TP.