【摘 要】
：
Intracellular delivery of functional molecules,such as DNA probes and plasmids,is an important method for investigating cellular mecha-nisms and changing cell fates in biomedicine.Among various delivery methods,recent years have seen the emergence of elec
【机 构】
：
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education,Beijing Advanced Innovat
论文部分内容阅读
Intracellular delivery of functional molecules,such as DNA probes and plasmids,is an important method for investigating cellular mecha-nisms and changing cell fates in biomedicine.Among various delivery methods,recent years have seen the emergence of electroporation-based techniques that provide versatile platforms for molecule delivery,with high efficiency and controlled dosage.In this Review,we describe recent electroporation-based systems for intracellular molecule delivery.The principles of electroporation for cell membrane perforation and cargo delivery are briefly summarized.Focusing on various scenarios for the application of electroporation,we review electroporation devices that variously employ structures based on nanochannels,nanostraws,and flow-through microfluidic channels for in vitro intracellular molecule delivery.We also consider in vivo targeted therapies based on delivery of active molecules by electroporation according to the lesion loca-tions.Finally,we discuss the current challenges facing electroporation-based techniques,as well as opportunities for their future development,which may lead to innovations in intracellular molecule delivery both for cellular analysis in the laboratory and treatment in the clinic.
其他文献
本文对781台铁塔安全监测系统的建设背景和系统主要功能进行了详细阐述.依托北京金纳科技有限公司在广播电视编解码、传输发射、融媒体演播室编辑制作、物联网技术开发、弱电工程、通信工程信息安全等领域的研发整合实力和丰富的施工经验,781台工程师深入剖析台站铁塔监测需求,提出高标准系统设计和施工要求,双方通力合作开发本系统,在实际应用中产生良好的监测效果,更好的保障了铁塔安全稳定和安全播出要求.
本文介绍了数字微波电路设备运行过程中出现的一些故障现象以及处理过程.
在有线电视传输系统中,分布连接着许多放大器,在放大器中的核心元件就是晶体管,工作时都会带来不同程度的噪声功率.所以,有线电视系统中的噪声是一个很重要的问题,必须设法加以控制.
广播电视作为党和政府的喉舌,担负着宣传党的路线、方针、政策的重要任务.安全播出工作是宣传工作的最终体现.“安全播出无小事”是宣传工作的重要前提,播出安全既是技术问题,也是政治问题,同时也是一个系统工程,本文从管理和技术两方面就如何做好安全播出工作进行了分析.
哈尔滨正泰广播设备有限公司生产的ZT-G1kW-Ⅲ型中波广播发射机由于技术成熟,运行可靠,故障率较低、维护简单等特点,深受广大基层台站喜爱.本文简要的介绍了哈尔滨正泰广播设备有限公司ZT-G1kW-Ⅲ型全固态中波广播发射机继电器控制板的自我保护功能,并对运行中出现的故障加以分析和处理.
Substantially lightweight brake discs with high wear resistance are highly desirable in the automotive industry.This paper presents an investigation of the precision-engineering design and development of automotive brake discs using nonhomogeneous A1/SiC
The mechanical behavior of individual cells plays an important role in regulating various biological activities at the molecular and cellular lev-els.It can serve as a promising label-free marker of cells\' physiological states.In the past two decades,s
PEDOT:PSS conductive polymers have received tremendous attention over the last two decades owing to their high conductivity,ease of processing,and biocompatibility.As a flexible versatile material,PEDOT:PSS can be developed into various forms and has had
The effects of the nonuniform cutting force and elastic recovery of processed materials in ultra-precision machining are too complex to be treated using traditional cutting theories,and it is necessary to take account of factors such as size effects,the u
Raman spectroscopy is a type of inelastic scattering spectroscopy that is widely used in determining and analyzing molecular struc-ture.It also has a number of practical applications in evaluating food safety,medicine,and forensics.The Raman spectral sig-