论文部分内容阅读
用盖玻片或ＭａｔＴｅｋ培养皿培养猪主动脉内皮细胞（ａＥＣ）约１８ｈ后，以荧光探针（ｆｌｕｏ－３／ＡＭ终浓度１０μｍｏｌ·Ｌ－１）负载，当细胞内游离钙离子（Ｃａ２＋）与ｆｌｕｏ－３结合后，通过激光扫描共聚焦显微镜（ＩｎＳＩＧＨＴ－ｐｌｕｓＩＱ型）观察细胞的荧光影像变化。基于三七总皂甙（ｔ－ＰＮＳ）对ａＥＣ所产生的生物活性物质有明显的作用，本拟进一步探索上述药物对ａＥＣ中［Ｃａ２＋］；的影响。实验结果显示：在ＡＴＰ作用后，ｔ－ＰＮＳ可使ａＥＣ内的荧光强度在２０～８０ｓ内急剧升高至峰值。但其组分Ｒｂ１及Ｒｇ１使荧光强度有下降或无明显变化。提示ｔ－ＰＮＳ有使内皮细胞［Ｃａ２＋］；升高的作用。
After aortic endothelial cells (aEC) were cultured with coverslips or MatTek petri dishes for approximately 18 h, they were loaded with a fluorescent probe (fluo-3/AM final concentration of 10 μmol·L-1) when intracellular free calcium ions (Ca2+) were present. After binding to fluo-3, the fluorescence changes of the cells were observed by a laser scanning confocal microscope (InSIGHT-plus IQ type). Based on the significant effect of panax notoginseng saponins (t-PNS) on the biologically active substances produced by aEC, the present study intends to further explore the effects of these drugs on [Ca2+] in aEC. The experimental results show that after ATP action, t-PNS can make the fluorescence intensity within aEC rise sharply within 20 ~ 80s to the peak. However, its components Rb1 and Rg1 decreased the fluorescence intensity or showed no significant change. Tip t-PNS has the effect of increasing endothelial [Ca2+];