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【目的】为了构建柳杉无性系的DNA指纹图谱,准确区分柳杉无性系。【方法】利用SSR标记分析了柳杉89个无性系的遗传多样性和指纹图谱。【结果】11对引物共检测出53个等位点,平均每个SSR位点有5个,变化范围为3~6个;有效等位基因数(Ne)变化范围为1.887 0~4.295 6,平均为3.041 1;Shannon信息指数(I)变化范围为0.774 9~1.556 3,平均为1.208 1。表明SSR标记具有较高的多态性。根据SSR标记特点及引物的顺序,将SSR引物扩增统计的“0”、“1”转换成基因型,通过不同基因型组合,构建了柳杉89个无性系的DNA分子指纹图谱,使每个无性系都获得了1个22位数的指纹图谱号码,同时基于个体间的遗传距离将柳杉89个无性系分为5类。【结论】SSR标记适用于柳杉无性系遗传多样性分析及鉴定,柳杉的遗传多态性处于中等偏高水平。
【Objective】 In order to construct the DNA fingerprinting of Cryptomeria fortunei clones, the cactus clones were accurately distinguished. 【Method】 The genetic diversity and fingerprints of 89 clones of Cryptomeria fortunei were analyzed by SSR markers. 【Result】 A total of 53 alleles were detected with 11 pairs of primers. The average number of SSR loci was 5, ranging from 3 to 6. The number of effective alleles (Ne) varied from 1.887 0 to 4.295 6, With an average of 3.041 1; Shannon’s information index (I) varied from 0.774 9 to 1.556 3 with an average of 1.208 1. The SSR markers showed high polymorphism. According to the characteristics of SSR markers and the order of primers, the “0 ” and “1” of the SSR primers were converted into genotypes. DNA fingerprinting of 89 clones of Cryptomeria fortunei was constructed by combining different genotypes At the same time, 89 clones of Cryptomeria fortunei were divided into 5 categories based on the genetic distance between individuals. 【Conclusion】 SSR markers are suitable for the analysis and identification of the genetic diversity of Cryptomeria fortunei clones. The genetic polymorphism of Cryptomeria fortunei is at a moderately high level.