Optimization of Purification of Flavonoids from Lophatherum gracile Brongn.

• 作 者: Yang CHU Qian YANG Mingyan JIANG
• 机 构: Department of Pharmacy,the First Affiliated Hospital of China Medical University,Shenyang Pharmaceut
• 出 处: Medicinal Plant
• 发表日期: 2013年05期
• 关键词: flavonoids effluent purchased ethanol purity systematically purification resin d

Objective To systematically study the factors which affect the purification of flavonoid from Lophatherum gracile Brongn.MethodHigh concentration of flavonoid extract from Lophatherum gracile Brongn.at pH 6.0 was eluted with water until the effluent was colorless,then 80% ethanol was used for elution at a rate of 2.0 mL /min until the effluent had no color reaction of flavonoids,then the eluent was collected.Result The average purity of flavonoids in solid product reached 57.5%.Conclusion This method is simple and feasible with good separation effect,which can meet the industrial requirement.

Objective To systematically study the factors which affect the purification of flavonoid from Lophatherum gracile Brongn. Method high concentration of flavonoid extract from Lophatherum gracile Brongn. At pH 6.0 was eluted with water until the effluent was colorless, then 80% ethanol was used for elution at a rate of 2.0 mL / min until the effluent had no color reaction of flavonoids, then the eluent was collected. Result The average purity of flavonoids in solid product reached 57.5% .Conclusion This method is simple and feasible with good separation effect, which can meet the industrial requirement.