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采用低压脉冲电泳转化仪转化外源基因pBI121进入水稻IR36部分酶解愈伤组织的细胞,培养分化再生植株(R_0),并获得转基因水稻植株的有性后代(R_1).对转化愈伤组织、转基因植株R_0及R_1的酶活性组织化学和分子杂交检测表明外源基因已通过有性过程遗传给后代,获得稳定表达.愈伤组织瞬间表达率为46.4％;R_0转化有效率为7.5％.
Low-voltage pulse electrophoresis (PCR) was used to transform exogenous gene pBI121 into partially digested callus of IR36 in rice, and the differentiated regenerated plants (R_0) were cultured and the progeny (R_1) of the transgenic rice plants were obtained. The enzymatic activities of R_0 and R_1 in transgenic plants were determined by histochemical and molecular hybridization tests.The result showed that the foreign gene was inherited to offspring through sexual process and was stably expressed.The transient expression rate of callus was 46.4% and the efficiency of R_0 transformation was 7.5%.