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用正、负高压静电场（HVEF），聚乙二醇（PEG），氯化钙（CaCl2）和二甲基亚矾（DMSO）等方法预处理大豆种子，比较种子抗低温吸胀冷害的能力结果表明：负高压静电场处理能促进膜相转变或损伤修复，并能在低温吸胀时减少无机离子，有机大分子物质的外渗；还能提高种子内自由基清除酶系的活性，促进糖原和贮存蛋白的代谢及生长调节物质的水平比较几种处理方法的效果表明：高压静电场处理主要提高自由基清除酶系和基础代谢酶的活性，而对吸胀时的膜损伤修复的促进效果较小；聚乙二醇和氯化钙则在促进种子吸胀时的睦相转变和膜损伤修复方面有显著作用所有的处理方法均在不同程度上抑制羟自由基（HO）的产生，从而减轻膜脂质过氧化的伤害.
Soybean seeds were pretreated with positive and negative high voltage electrostatic field (HVEF), polyethylene glycol (PEG), calcium chloride (CaCl2) and dimethyl sulfoxide (DMSO) The results show that the negative high voltage electrostatic field can promote the phase transition or damage repair, and can reduce the extravasation of inorganic ions and organic macromolecules during the low temperature swelling. It can also increase the activity of free radical scavenging enzymes in seeds and promote Metabolism of glycogen and storage protein and the level of growth-regulating substances Compared with several treatment methods, the results show that: high-voltage electrostatic field treatment mainly increases the activity of free radical scavenging enzymes and basal metabolic enzymes, while the membrane damage during intumescent repair The effect of PEG and CaCl2 was significant in promoting the phase transition and membrane damage repair during seed absorption. All treatments inhibited the production of hydroxyl free radicals (HO) to varying degrees, Thereby reducing the membrane lipid peroxidation damage.