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目的:检测肠易激综合征(IBS)模型大鼠肺组织P物质(SP)表达变化,探讨中医肺与大肠相表里脏腑相关理论的科学内涵。方法:采用慢性应激刺激与束缚相结合的方法建立IBS模型大鼠,复制成功后分别以免疫组化、酶联免疫吸附试验(ELISA)和逆转录多聚酶链反应(RT-PCR)等方法检测肺组织中的P物质(SP)表达。结果:与对照组比较IBS模型组大鼠的肺组织中SP含量以及基因表达明显增高(P<0.01)。结论:IBS状态下大鼠肺组织中SP的含量及基因表达发生上调,提示肺与大肠通过神经-内分泌系统存在着内在联系。
Aims: To detect the expression of substance P (SP) in the lung tissue of rats with irritable bowel syndrome (IBS) and to explore the scientific connotation of related theory of viscera in lung and large intestine. Methods: IBS model rats were established by the combination of chronic stress stimulation and restraint. After successful replication, they were detected by immunohistochemistry, enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) P substance (SP) expression in lung tissue. Results: Compared with control group, SP content and gene expression in lung tissue of IBS model group were significantly increased (P <0.01). CONCLUSIONS: The content and gene expression of SP in rat lung tissue under the condition of IBS are up-regulated, suggesting that there is an internal relationship between the lung and the large intestine through the neuroendocrine system.