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目的观察西酞普兰对胎鼠海马神经前体细胞(NPC)增殖和分化的影响。方法分离孕14.5d 胎鼠海马,有限稀释法进行单克隆体外培养,分为不同浓度西酞普兰组和对照组。分别用无血清 NPC 培养基和胎牛血清促其增殖和诱导分化。四甲基氮唑蓝(MTT)法和免疫荧光法分别观察不同浓度西酞普兰对 NPC 增殖和分化的影响。结果 MTT 结果显示西酞普兰(1.0μmol/L)干预后第3天至第7天,吸光度(A_(570 nm))显著增高(均 P<0.0001);西酞普兰(0.5μmol/L、1.0μmol/L)干预7d 后,NeuN 阳性细胞率分别达59.5%±2.8%、57.8%±2.1%,均高于对照组的47.3%±4.3%(均 P<0.05)。结论西酞普兰促进胎鼠 NPC 的增殖和向神经元的分化。
Objective To observe the effects of citalopram on the proliferation and differentiation of fetal rat hippocampal neural precursor cells (NPCs). Methods Fetal rat hippocampus was isolated from pregnant 14.5 days embryos and cultured in vitro by limiting dilution method. Cilostazol was divided into different concentrations of citalopram and control group. Respectively with serum-free NPC medium and fetal bovine serum to promote proliferation and differentiation. Tetramethylpyrazolyl blue (MTT) and immunofluorescence were used to observe the effects of citalopram at different concentrations on the proliferation and differentiation of NPC. Results The MTT results showed that the absorbance (570 nm) was significantly increased on the 3rd day to the 7th day after treatment with citalopram (1.0 μmol / L) (all P <0.0001) μmol / L) for 7 days, the positive rate of NeuN positive cells were 59.5% ± 2.8% and 57.8% ± 2.1%, respectively, which were all higher than those in the control group (47.3% ± 4.3%, P <0.05). Conclusion Citalopram promotes the proliferation and differentiation of fetal rat NPC to neurons.