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为探讨金黄米抗氧化活性,对其5个粗提取物进行体外清除DPPH自由基抗氧化活性和体内动物试验,并对分离得到的提取化合物进行OH·与O2-·清除ESR抗氧化试验。结果表明:总提取物、石油醚提取物、氯仿提取物、正丁醇提取物、水提取物对DPPH清除能力IC50分别为0.69、1.03、0.74、0.11、0.17mg/mL,其中正丁醇提取物活性最高,是α-生育酚的81%。在1mg/mL质量浓度下,对OH·的清除能力,龙胆苦苷的清除率为75%,黄花远志素A的清除率为62.5%。对O2·-的清除能力,龙胆苦苷的清除率达到59.9%,表明龙胆苦苷是金黄米抗氧化的主要活性物质。金黄米提取物可显著提高衰老小鼠血清和肝脏的超氧化物岐化酶和谷胱甘肽过氧化物酶活力,降低脂质过氧化物丙二醛含量;可上调抗氧化酶mRNA的表达。
In order to explore the antioxidative activity of golden rice, five crude extracts were screened for DPPH free radical antioxidant activity and in vivo animal experiments. The isolated compounds were also subjected to OH · and O2- · scavenging ESR antioxidant tests. The results showed that the IC50 of total extract, petroleum ether extract, chloroform extract, n-butanol extract and water extract on DPPH were 0.69,1.03,0.74,0.11,0.17mg / mL respectively, The highest activity, is 81% of α-tocopherol. At a concentration of 1 mg / mL, the scavenging capacity of gentiopicroside was 75% for OH · and 62.5% for nerolidin A. Clearing capacity of O2 · -, gentiopicroside clearance rate of 59.9%, indicating that gentiopicroside is the main antioxidant activity of golden yellow rice. Golden Rice Extract can significantly increase superoxide dismutase and glutathione peroxidase activities in serum and liver of senile mice and decrease lipid peroxides malondialdehyde; up-regulate the expression of anti-oxidase mRNA .