论文部分内容阅读
本工作观察注射利血平对LH-RH 终止妊娠作用的影响,并对其作用机理进行初步分析。将36只妊娠大鼠随机分成三组进行实验,即盐水对照组,LH-RH 组和LH-RH 加利血平组。分别于妊娠的第5—7天给予不同的处理。于妊娠的第17天处死,观察妊娠胎数、称卵巢和垂体重量,同时测定卵巢3β-羟甾脱氢酶的活性及血清申孕酮(孕9、13、17)和雌二醇的含量变化。结果发现对照组10只动物全部妊娠,LH-RH 组15只动物全部流产,而LH-RH 加利血平组11只动物也全部妊娠,胚胎发育正常。由此可见,利血平有拮抗LH-RH的致流产作用。我们还发现,注射LH-RH 类似物后,血清中孕酮、雌二醇含量及卵巢重量、卵巢△~5-3β羟甾脱氢酶的活性比对照组明显降低,而同时给予利血平后可以阻止血清孕酮、雌二醇含量、卵巢重量的降低,并使△~5-3β羟甾脱氢酶活性超过正常。这一结果一方面提示LH-RH 的致流产作用极可能是由于减少了内源性孕酮和雌二醇作用的结果;另一方面也提示利血平对大剂量LH-RH 类似物终止妊娠的拮抗作用很可能是通过垂体及卵巢两方面加强和促进甾体激素的合成而实现的。
This work observed injection of reserpine on the termination of pregnancy LH-RH effect, and its mechanism of action for a preliminary analysis. Thirty-six pregnant rats were randomly divided into three groups: saline control group, LH-RH group and LH-RH galectin group. Different treatments were given on days 5-7 of pregnancy. Were sacrificed on the 17th day of gestation to observe the gestational age, the ovary and the pituitary weight, the activity of 3β-hydroxy steroid dehydrogenase in the ovary and the serum progestin (pregnant 9, 13, 17) and estradiol Variety. The results showed that 10 animals in the control group were all pregnant. All 15 animals in the LH-RH group were aborted, while 11 animals in the LH-RH and the reserpine group were all pregnant and had normal embryonic development. Thus, reserpine antagonistic LH-RH induced abortion. We also found that the serum progesterone, estradiol content and ovarian weight, ovarian △ 5-3β-hydroxy-steroid dehydrogenase activity was significantly lower than the control group after injection of LH-RH analogues, while reserpine Can prevent serum progesterone, estradiol levels, decreased ovarian weight, and △ △ 5-3β hydroxy steroid dehydrogenase activity than normal. This result, on the one hand, suggests that LH-RH induced abortion is most likely due to reduced endogenous progesterone and estradiol effects; on the other hand, it also suggests that reserpine inhibits high-dose LH-RH analogue termination of pregnancy Antagonism is likely to be achieved through both the pituitary and ovary to enhance and promote the synthesis of steroid hormones.