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对蛋白酶水解鸡卵清蛋白获得的肽类物质的理化特性及对免疫功能的调节作用进行研究。方法为凯氏定氮法测定蛋白质含量。多肽含量测定,首先经三氯醋酸沉淀样品中大分子蛋白质,按凯氏定氮法测定上清液的多肽含量。HPLC色谱法测定肽的分子量范围。免疫功能检查包括细胞免疫和体液免疫两项功能测定。与实验对照组比较,迟发型变态反应实验、小鼠半数溶血值测定实验、小鼠巨噬细胞吞噬鸡红细胞实验及NK细胞活性测定实验均有显著增强作用。提示卵清蛋白肽对实验小鼠的机体具有免疫增强作用。
The physical and chemical properties of peptides obtained from protease hydrolysis of chicken ovalbumin and the regulation of immune function were studied. Methods Kjeldahl method for the determination of protein content. Peptide content determination, first by trichloroacetic acid precipitation of the sample of macromolecular protein, according to Kjeldahl determination of the polypeptide content of the supernatant. HPLC chromatographic determination of the molecular weight range of the peptide. Immune function tests include both cellular and humoral immune function tests. Compared with the experimental control group, late-onset allergic reaction test, hemolytic half-value test, macrophage phagocytosis of chicken erythrocytes and NK cell activity assay were all significantly enhanced. Tip ovalbumin peptide on the experimental mice with immune enhancement.