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葡萄糖对卵裂阶段胚胎的发育有抑制作用，但早期胚胎体外发育中添加葡萄糖并不是完全不必要的，8-细胞前需要非必需氨基酸，8-细胞及以后利用所有氨基酸。低浓度必需氨基酸促进胚胎发育，高浓度必需氨基酸抑制胚胎发育。牛血清白蛋白等大分子物质既能供给胚胎营养，促进细胞生长、增殖与分裂，又能消除血清的不利影响，是很好的蛋白质替代品，一些生长因子对胚胎发育起积极作用。共培养及序贯培养虽可以改善胚胎发育状况，但也存在一些负面影响。“,”Glucose has inhibitory effect on embryos development in cleavage stage ,but it is unnecessary to add glucose in early embryos development in vitro, embryos before stage of 8-cells need non-essential amino acid,embryos of and after stage of 8-cells need all amino acids.Low concentration of essential amino acids can promote the embryonic development, while high concentration of essential amino acids inhibit embryonic development.Bovine serum albumin and other macromolecules can supply nutrition to the embry-os,as well as promote cell growth,proliferation,differentiation,and eliminate the adverse effects of serum, which makes them very good protein substitutes.Many growth factors also have positive effects on embryonic development.Coculture and sequential culture can improve the status of embryonic development, although there are also some negative effects.