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为了更准确、有效地揭示油菜资源遗传多样性,探索SSR和SRAP 2种分子标记在油菜菌核病抗性资源遗传多样性分析中的应用,采用40对SSR核心引物及陕西理工学院生物学院分子与遗传实验室筛选出的40对多态性高、条带清晰的SRAP引物,对陕西省汉中市农科所经过连续3年牙签茎秆接种试验结合多年的田间抗性表现筛选出的43份菌核病抗性较好的油菜材料进行遗传多样性分析,并对2种分子标记揭示的多态性条带数、多态性信息含量(PIC)进行比较。结果表明:2种标记共检测出634个条带,SRAP标记检测的多态性条带数(335)较SSR(287)高,而SSR引物的平均多态性信息含量(PIC)值较SRAP引物高,分别是0.76和0.69。在遗传相似系数0.67处,43份油菜材料被分为Ⅲ类,白菜型油菜(丰油10号白菜型选系)可较好地与甘蓝型油菜区分。主成分分析(Principal component analysis,PCA)、群体结构分析与聚类分析结果相似,说明SSR与SRAP标记结合能准确有效的反映油菜材料的亲缘关系。供试43份油菜材料遗传相似系数分布在0.65~0.81,表明遗传相似性较高,亲缘关系较近。因此,应进一步加强抗源筛选及引进,对现有材料进行遗传改良,拓宽其遗传背景,从而为抗菌核病油菜品种选育奠定基础。
In order to reveal the genetic diversity of rapeseed more accurately and effectively, the application of SSR and SRAP markers in the analysis of genetic diversity of sclerotinia sclerotiorum resources was explored. Forty pairs of SSR core primers and molecular markers of Shaanxi Institute of Technology Forty-six pairs of SRAP primers with high polymorphism and clear bands were screened from genetic laboratory and screened 43 years from field-resistance test of peanut in Hanzhong City of Shaanxi Province for three years in succession The genetic diversity of rapeseed with better resistance to sclerotinia was analyzed, and the number of polymorphic bands and polymorphism information (PIC) revealed by two kinds of molecular markers were compared. The results showed that 634 bands were detected by the two markers, the number of polymorphic bands (335) detected by SRAP markers was higher than that of SSR (287), while the average polymorphism information content (PIC) Primer high, respectively 0.76 and 0.69. At the genetic similarity coefficient of 0.67, 43 rapeseed materials were divided into three categories. Brassica napus (Feng-yu-10 cabbage-type selection line) could be distinguished from Brassica napus. Principal component analysis (PCA) and population structure analysis are similar to the results of cluster analysis, indicating that SSR and SRAP markers can accurately and effectively reflect the genetic relationship of rapeseed. The genetic similarity coefficients of 43 tested rapeseed cultivars ranged from 0.65 to 0.81, indicating that the genetic similarity was high and the genetic relationship was relatively close. Therefore, it is necessary to further strengthen screening and introduction of anti-source, genetic improvement of existing materials and broaden their genetic background, so as to lay the foundation for the breeding of rape varieties resistant to Sclerotinia sclerotiorum.