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采用胰管注入2.5％去氧胆酸钠—胰蛋白酶溶液造成大白鼠急性出血性胰腺炎模型,动态观察其血清淀粉酶活性及淀粉酶同功酶酶谱。结果显示:急性胰腺炎大鼠在发病后第一天,血清淀粉酶活性明显增高;血清中既有S型,也有P型淀粉酶同功酶出现,且P型随炎症的消退而减少至消失。在正常的鼠血清中仅看到S型淀粉酶同功酶。实验结果提示:检测血清淀粉酶同功酶,不仅对诊断大白鼠实验性胰腺炎是一特异性的指标,且还可通过动态观察其变化,了解该炎症病变的发展。
Pancreatic duct was injected into 2.5% sodium deoxycholate-trypsin solution to induce acute hemorrhagic pancreatitis in rats, and serum amylase activity and amylase isoenzymatic spectrum were dynamically observed. The results showed that the activity of serum amylase increased significantly in rats with acute pancreatitis on the first day after onset; there were both S-type and P-amylase isoenzymes in serum, and P-type decreased to disappear with the disappearance of inflammation. . Only S-type amylase isozymes are seen in normal mouse serum. The experimental results suggest that the detection of serum amylase isoenzyme is not only a specific marker for the diagnosis of experimental pancreatitis in rats, but also the dynamic observation of the changes to understand the development of the inflammatory lesions.