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目的:探讨具有亲表皮现象的疾病T细胞受体γ链基因重排的情况。方法:用免疫组化标记筛选17例T细胞淋巴瘤、30例可疑为T细胞淋巴瘤和10例副银屑病,采用聚合酶链式反应扩增方法检测T细胞受体γ链基因重排。结果:17例T细胞淋巴瘤中12例出现T细胞受体γ链基因重排,30例可疑为T细胞淋巴瘤和10例副银屑病均无T细胞受体γ链基因重排。结论:T细胞受体γ链基因重排检测是区分T淋巴细胞浸润的亲表皮疾病鉴别诊断的有效方法,且对T细胞淋巴瘤的确诊有重要参考价值与显著意义。
Objective: To investigate the rearrangement of γ-chain of T cell receptor with disease of pro-epidermis. Methods: 17 cases of T-cell lymphoma, 30 cases of suspected T-cell lymphoma and 10 cases of paranoid psoriasis were screened by immunohistochemistry. The T-cell receptor γ-chain gene rearrangements were detected by polymerase chain reaction amplification . RESULTS: T-cell receptor γ-chain gene rearrangements occurred in 12 out of 17 T-cell lymphomas. T cell receptor γ-chain rearrangements were not found in 30 suspected T-cell lymphoma and 10 paraneoplastic psoriasis. CONCLUSION: T-cell receptor γ-chain gene rearrangement detection is an effective method to differentiate pro-epidermal diseases in which T-lymphocytes infiltrate. It also has important reference value and significance for the diagnosis of T-cell lymphoma.